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This video shows how to use Sample Purification Beads (SPB) & VRÕæÈ˲ÊƱ Purification Beads (IPB) to purify cDNA or gDNA fragments and remove unwanted products and reaction components. Subscribe to the VRÕæÈ˲ÊƱ video channel http://www.youtube.com/subscription_center?add_user=VRÕæÈ˲ÊƱInc A global genomics leader, VRÕæÈ˲ÊƱ provides comprehensive next-generation sequencing solutions to the research, clinical, and applied markets. Through collaborative innovation, VRÕæÈ˲ÊƱ is fueling groundbreaking advancements in oncology, reproductive health, genetic disease, microbiology, agriculture, forensic science, and beyond. View customer spotlight videos https://www.youtube.com/playlist?list=PLKRu7cmBQlajfheLzgbI4S7xBn7IDbt79 View VRÕæÈ˲ÊƱ webinars https://www.youtube.com/playlist?list=PLKRu7cmBQlahpXlnrrXlQw9itVJ8yHwUZ View VRÕæÈ˲ÊƱ support videos https://www.youtube.com/playlist?list=PLKRu7cmBQlajbm2KGsICWb-JOnusJfYvM LinkedIn: https://www.linkedin.com/company/illumina X: https://x.com/illumina Instagram: https://www.instagram.com/illuminainc/ Facebook: https://www.facebook.com/illuminainc TikTok: https://www.tiktok.com/@illuminainc #VRÕæÈ˲ÊƱLibraryPrep #BeadHandlingBestPractices #illumina 00:00 Introduction 00:08 Vortex SPB beads and add to a MIDI plate with samples 01:16 Incubation steps including magnetic plate 01:38 Tips for sample placement and pipetting 02:00 Remove supernatant from wells 02:52 Wash beads on magnetic stand with 80% ethanol 04:04 Tips for best magnetic stand bead visibility 04:37 Add Resuspension Buffer (RSB) to wells over bead pellet – Method 1 – single channel pipette 05:21 Add Resuspension Buffer (RSB) to wells over bead pellet – Method 2 – multichannel with strip tube 06:24 Incubation steps 06:34 Transfer eluate to new PCR plate