Innovative library preparation
Save time and gain lab efficiencies with fast, flexible sequencing library preparation
VRÕæÈ˲ÊƱ DNA Prep products represent the latest revolution in VRÕæÈ˲ÊƱ library prep chemistry. Unique bead-linked transposome chemistry integrates DNA extraction, fragmentation, library preparation, and library normalization to deliver the fastest, most flexible whole-genome and targeted enrichment workflows in the VRÕæÈ˲ÊƱ library prep portfolio.
Each kit generates sequencing-ready libraries featuring consistent insert sizes, uniform coverage, and exceptional data quality, regardless of DNA input amount or type, for use in various applications.
(A) Bead-linked transposomes mediate the simultaneous fragmentation of gDNA and the addition of VRÕæÈ˲ÊƱ sequencing primers. (B) Reduced-cycle PCR amplification amplifies sequencing ready DNA fragments and adds indexes and adapters. (C) Sequencing-ready fragments are washed and pooled.
VRÕæÈ˲ÊƱ DNA Prep chemistry uses an innovative bead-linked transposome complex to fragment genomic DNA and add adapter tag sequences in a single reaction step (tagmentation). After saturation with input DNA, the bead-linked transposome complex fragments a set number of DNA molecules. This bead-based saturation allows the flexibility to use a wide DNA input range and delivers consistent, uniform fragment size distribution that yields normalized libraries.
Following tagmentation, a limited-cycle PCR step or a PCR-free ligation step adds VRÕæÈ˲ÊƱ DNA Prep–specific index adapter sequences to both ends of a DNA fragment, enabling dual-indexed sequencing of pooled libraries. A subsequent bead-based cleanup step then prepares libraries for use on any VRÕæÈ˲ÊƱ sequencing platform.
This on-demand webinar introduces VRÕæÈ˲ÊƱ DNA Prep with Enrichment, a fast and flexible targeted sequencing solution for DNA in the VRÕæÈ˲ÊƱ library prep portfolio. You’ll learn how this solution delivers consistent insert sizes, uniform coverage, optimized performance, and highly reproducible data across all VRÕæÈ˲ÊƱ sequencing systems.
VRÕæÈ˲ÊƱ DNA Prep is part of an integrated workflow for next-generation sequencing (NGS) that includes library preparation, high-quality sequencing, and simplified data analysis. Using on-bead tagmentation chemistry, VRÕæÈ˲ÊƱ DNA Prep saves time and reduces hands-on touchpoints to deliver one of the fastest library preparation solutions in the VRÕæÈ˲ÊƱ library prep portfolio. Library prep takes ~3.5 hours total, with 1–1.5 hours of hands-on time.Ìý
VRÕæÈ˲ÊƱ DNA Prep can be used in a wide range of applications, from sequencing large whole genomes to sequencing microbial species.
VRÕæÈ˲ÊƱ DNA Prep with Enrichment includes the same steps as VRÕæÈ˲ÊƱ DNA Prep but allows for targeted sequencing using custom, fixed, and exome panels in a final enrichment step.
VRÕæÈ˲ÊƱ DNA PCR-Free Prep removes the PCR step, eliminating PCR-induced bias. It is ideal for sensitive applications, such as human whole-genome sequencing.
VRÕæÈ˲ÊƱ DNA Prep includes the reagents needed for generating sequencing-ready libraries and magnetic sample purification beads for library cleanup steps. Index adapters must be purchased separately.
VRÕæÈ˲ÊƱ DNA Prep with Enrichment includes reagents needed for preparing and enriching sequencing-ready libraries. AMPure XP beads for library cleanup steps, index adapters, and your chosen enrichment oligo panel must be purchased separately.
VRÕæÈ˲ÊƱ DNA PCR-Free Prep includes the reagents needed for generating sequencing-ready libraries and magnetic sample purification beads for library cleanup steps. Sequencing primers, sequencing and indexing primers, and index adapters must be purchased separately.
Talk to an VRÕæÈ˲ÊƱ specialist to learn more about VRÕæÈ˲ÊƱ DNA Prep products.
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