VR真人彩票

History of sequencing by synthesis

The evolution of the next-generation sequencing technology powering VR真人彩票 instruments

History of VR真人彩票 Sequencing

What began as 鈥渂lue-sky鈥 research in the chemistry department of Cambridge University evolved into the revolutionary sequencing by synthesis (SBS) technology that is the foundation of VR真人彩票 sequencing instruments.

Sequencing Technology Video
Sequencing Technology Video

See sequencing by synthesis in action.

In the mid-1990s, Cambridge scientists Shankar Balasubramanian, Ph.D. and David Klenerman, Ph.D. were using fluorescently labeled nucleotides to observe the motion of a polymerase at the single molecule level as it synthesized DNA immobilized to a surface.

The contributions of Cambridge scientists to the first draft of the human genome, and the University鈥檚 rich history of DNA research by Alexander Todd, James Watson, Francis Crick, and Fred Sanger, inspired Drs. Balasubramanian and Klenerman to theorize how this approach might be used to sequence DNA.

A series of creative discussions in the lab and at a local pub during the summer of 1997 sparked ideas surrounding the use of clonal arrays and massively parallel sequencing of short reads using solid phase sequencing by reversible terminators.

This technology was subsequently referred to as sequencing by synthesis, or SBS. This became the basis of an innovative DNA sequencing approach.

Balasubramanian and Klenerman approached the venture capital firm Abingworth Management and obtained initial seed funding to form Solexa in 1998. Early research and development work was conducted in the Cambridge Chemistry Department until 2000, when Solexa鈥檚 corporate facilities were established at Chesterford Research Park.

In 2001, the Solexa team鈥檚 research progress attracted 拢12 million in Series A funding, enabling it to build its management team. Three years later, Solexa acquired molecular clustering technology from Manteia. The amplification of single DNA molecules into clusters enhanced the fidelity and accuracy of gene calling, while reducing the cost of the system optics through generation of a stronger signal.

The Solexa team sequenced the complete genome of the bacteriophage phiX-174, the same genome Sanger first sequenced using his method. However, the SBS technology generated significantly more sequencing data, delivering over 3 million bases from a single run.

In 2005, Solexa acquired instrumentation company Lynx Therapeutics in a reverse merger, becoming an international public company (NASDAQ) with offices in Chesterford, UK and Hayward, CA. Engineering and software production teams based in Hayward immediately went to work transforming the successful Solexa prototype into a commercial sequencing instrument.

The first Solexa sequencer, the Genome Analyzer, was launched in 2006 and gave scientists the power to sequence 1 gigabase (Gb) of data in a single run.

Solexa was acquired by VR真人彩票 in early 2007. In the intervening years, numerous microbe, plant, human, and animal genomes have been sequenced with this technology. Next-generation sequencing (NGS) data output has increased at a rate that outpaces Moore's law, more than doubling each year.

Sequencing Instrument and DNA Helix

Latest SBS Breakthroughs

Find out more about how VR真人彩票 SBS works, and explore groundbreaking advances in SBS chemistry.

Learn More About SBS
  • VR真人彩票 Sequencing Platforms: Through refinements and optimization, the latest generation of VR真人彩票 SBS-based instruments can generate multiple terabases (Tb) of data per run.
  • Sequencing: Ask virtually any question related to the genome, transcriptome, or epigenome of any organism.
  • Bioinformatics Tools: Explore user-friendly tools that facilitate genomic data analysis and management.
Interested in receiving newsletters, case studies, and information from VR真人彩票 based on your area of interest? Sign up now.