| Read length | MiniSeq System Mid Output Kit | MiniSeq System Rapid Kit | MiniSeq System High Output Kit |
|---|---|---|---|
| 1 × 75 bp | N/A | N/A | 1.65–1.875 Gb |
| 2 × 75 bp | N/A | N/A | 3.3–3.75 Gb |
| 1 × 100 bp | N/A | 2 Gb | N/A |
| 2 × 150 bp | 2.1–2.4 Gb | N/A | 6.6–7.5 Gb |
MiniSeq Sequencing System specifications
Robust sequencing with a small footprint
Rapid turnaround, scalable throughput, and cost-effective benchtop sequencing with high quality scores
For every lab, everywhere
Access NGS with unrivaled simplicity and unthinkable speed
Output per flow cell for various read lengthsa
MiniSeq Sequencing System
a. Install specifications based on VRÕæÈ˲ÊƱ PhiX control library at supported cluster densities (129 –165 k/mm2 clusters passing filter). Actual performance parameters may vary based on sample type, sample quality, and clusters passing filter. All MiniSeq System kits are paired-end compatible.
Reads passing filter per flow cell
MiniSeq Sequencing System
| Read length | MiniSeq System Mid Output Kit | MiniSeq System Rapid Kit | MiniSeq System High Output Kit |
|---|---|---|---|
| Single-end reads | 7–8²Ñ | 20M | 22–25²Ñ |
| Paired-end reads | 14–16²Ñ | N/A | 44–50²Ñ |
Quality scoresa
MiniSeq Sequencing System
| Read length | MiniSeq System Mid Output Kit | MiniSeq System Rapid Kit | MiniSeq System High Output Kit |
|---|---|---|---|
| 1 × 75 bp | N/A | N/A | > 85% bases higher than Q30Ìý |
| 2 × 75 bp | N/A | N/A | > 85% bases higher than Q30Ìý |
| 1 × 100 bp | N/A | > 85% bases higher than Q30 | N/A |
| 2 × 150 bp | > 80% bases higher than Q30 | N/A | > 80% bases higher than Q30 |
a. A quality score is a prediction of the probability of an error in base calling. The percentage of bases > Q30 is averaged across the entire run.
Run timea
MiniSeq Sequencing System
| Read length | MiniSeq System Mid Output Kit | MiniSeq System Rapid Kit | MiniSeq System High Output Kit |
|---|---|---|---|
| 1 × 75 bp | N/A | N/A | 7 hr |
| 2 × 75 bp | N/A | N/A | 13 hr |
| 1 × 100 bp | N/A | < 5 hr | N/A |
| 2 × 150 bp | ~17 hr | N/A | ~24 hr |
a. Includes cluster generation, sequencing, and base calling with quality scores on a MiniSeq System. Run times are based on VRÕæÈ˲ÊƱ PhiX control library at supported cluster densities. Performance may vary based on library type and quality, insert size, loading concentration, and other experimental factors.
Instrument specifications
MiniSeq instrument specifications
-
Light emitting diode (LED)
WavelengthsGreen 510-525 nm
Red 645-655 nm -
Dimensions
W x D x H45.6 cm × 48 cm × 51.8 cm
(18.0 in × 18.9 in × 20.4 in) -
Weight
45 kg (99 lb)
-
Crated weight
56.5 kg (125 lb)
Key technologies
Sequencing by synthesis chemistry
The MiniSeq System harnesses proven VRÕæÈ˲ÊƱ sequencing by synthesis (SBS) chemistry to deliver highly accurate data and robust performance for a broad range of applications. SBS chemistry uses reversible-terminator fluorescently labeled nucleotides to detect single bases as they are incorporated into growing DNA strands, reading billions of sequences in parallel.
2-channel sequencing by synthesis
The MiniSeq System leverages 2-channel sequencing by synthesis (SBS) for faster data generation than the original VRÕæÈ˲ÊƱ 4-channel SBS technology, with the same high data accuracy.