VRÕæÈ˲ÊƱ

Library Preparation
DNA Sequencing RNA Sequencing Methylation Sequencing Library Preparation High-Throughput Sequencing

Library prep automation

Minimize errors, reduce hands-on-time, and enable higher throughput with automation options across VRÕæÈ˲ÊƱ library prep products

Scientist starting library prep automation sequence

Automate your library prep

Automated liquid-handling systems help labs achieve and maintain consistent results while driving efficiency. NGS library prep automation also allows for the rapid scaling of throughput while reducing hands-on time.

VRÕæÈ˲ÊƱ partners with leading vendors to provide automated protocols for VRÕæÈ˲ÊƱ library prep kits, combining our partners’ automation knowledge with our library prep and sequencing expertise to build an NGS solution that works for you.

Library Prep Automation Buyer’s Guide

This guide walks you through the range of automation tools available for NGS library prep and helps you find a solution tailored for your application, throughput, and budget needs. Learn about top considerations for building your automated library prep protocol.

Types of library prep automation protocols

VRÕæÈ˲ÊƱ Qualified

Methods are developed by our automation partners with input from VRÕæÈ˲ÊƱ. VRÕæÈ˲ÊƱ reviews sequencing data from these methods to ensure performance and data quality. Our partners own, supply, install, and support these methods. Our partners service and support their liquid handler systems.

VRÕæÈ˲ÊƱ Qualified Plus

VRÕæÈ˲ÊƱ co-develops and tests the method with our automation partners. Our partners own, supply, and install these methods. VRÕæÈ˲ÊƱ provides front-line field and technical support for these methods.* Our partners provide secondary support for these methods. Our partners service and support their liquid handler systems.

Partner-developed

These methods are developed and tested by our automation partners. VRÕæÈ˲ÊƱ does not review the data from these methods. Our partners own, install, and support these methods. Our partners service and support their liquid handler systems.

*Support levels may vary by region. Contact your local support team for more information.

Compatible automation platforms for VRÕæÈ˲ÊƱ library preparation

See which library prep kits are automated on which liquid handling platforms, or find VRÕæÈ˲ÊƱ Qualified and/or partner-developed methods for a given platform.

  • VRÕæÈ˲ÊƱ Qualified
  • VRÕæÈ˲ÊƱ Qualified Plus
Ìý
AmpliSeq for VRÕæÈ˲ÊƱ**
  • Biomek i5
  • epMotion 5075t
  • epMotion 5073
 NGS STAR
NGS STARlet
  • Sciclone G3 NGSx
 Ìý
AmpliSeq for VRÕæÈ˲ÊƱ Cancer HotSpot Panel v2**
  • Biomek i5
Biomek NGeniuS
  • epMotion 5075t
  • epMotion 5073
 Ìý Sciclone G3 NGSx Ìý
AmpliSeq for VRÕæÈ˲ÊƱ Comprehensive Panel v3**
  • Biomek i5
 Ìý Ìý Ìý Ìý
AmpliSeq for VRÕæÈ˲ÊƱ Focus Panel**
  • Biomek i5
  • epMotion 5075t
  • epMotion 5073
 Ìý Sciclone G3 NGSx Ìý
COVIDSeq Assay (96 samples)Ìý Biomek i7 epMotion 5075t NGS STAR
  • Sciclone G3 NGSx
Sciclone G3 NGSx iQ
Zephyr G3 NGS		 Fluent DreamPrep
COVIDSeq Test (RUO Version) Ìý epMotion 5075t NGS STAR
  • Sciclone G3 NGSx
Sciclone G3 NGSx iQ
Zephyr G3 NGS		 Fluent DreamPrep
VRÕæÈ˲ÊƱ Complete Long Read Prep, Human Ìý epMotion 5075t Ìý
  • Sciclone G3 NGSx
 Ìý
VRÕæÈ˲ÊƱ DNA PCR-Free Prep Biomek i5
  • Biomek i7
  • Biomek NGeniuS
  • epMotion 5075t
  • NGS STAR
NGS STARlet		 Fontus NGS
  • Sciclone G3 NGSx
Sciclone G3 NGSx iQ
  • DreamPrep
VRÕæÈ˲ÊƱ DNA Prep Biomek i5
  • Biomek i7
  • Biomek NGeniuS
  • epMotion 5075t
  • NGS STAR
  • Sciclone G3 NGSx
Sciclone G3 NGSx iQ
Zephyr G3 NGS
  • DreamPrep
Freedom EVO NGS
VRÕæÈ˲ÊƱ DNA Prep with Exome 2.0 Plus Enrichment
  • Biomek i7
  • epMotion 5075t
  • NGS STAR
NGS STARlet
  • Sciclone G3 NGSx
Sciclone G3 NGSx iQ		 DreamPrep
VRÕæÈ˲ÊƱ DNA Prep with Exome 2.5 Enrichment Ìý
  • epMotion 5075t
 NGS STAR
  • NGS STARlet
  • Sciclone G3 NGSx
  • Sciclone G3 NGSx iQ
 Ìý
VRÕæÈ˲ÊƱ RNA Prep with Enrichment Biomek i5
  • Biomek i7
  • Biomek NGeniuS
  • epMotion 5075t
 NGS STAR
  • Sciclone G3 NGSx
Sciclone G3 NGSx iQ		 DreamPrep
VRÕæÈ˲ÊƱ Stranded mRNA Prep
  • Biomek i7
  • epMotion 5075t
  • STAR MOA
  • Sciclone G3 NGSx
Sciclone G3 NGSx iQ		 DreamPrep
llumina Stranded Total RNA Prep Ìý
  • epMotion 5075t
  • NGS STAR
  • Sciclone G3 NGSx
Sciclone G3 NGSx iQ		 DreamPrep
Nextera XT DNA
  • Biomek i7
  • epMotion 5075t
  • NGS STAR
  • Sciclone G3 NGSx
Sciclone G3 NGSx iQ
Zephyr G3 NGS		 DreamPrep
Pillar Biosciences oncoReveal Solid Tumor v2 Gene Panel Kit
  • Biomek NGeniuS
 Ìý Ìý Ìý Ìý
TruSeq DNA Nano
  • Biomek i7
  • epMotion 5075t
  • NGS STAR
 Sciclone G3 NGSx
  • Freedom EVO NGS
TruSeq DNA PCR-Free
  • Biomek i5
  • Biomek i7
  • epMotion 5075t
 NGS STAR
  • Sciclone G3 NGSx
Sciclone G3 NGSx iQ
  • Freedom EVO NGS
DreamPrep
TruSeq Stranded mRNA
  • Biomek i7
  • epMotion 5075t
  • NGS STAR
  • Sciclone G3 NGSx
Sciclone G3 NGSx iQ
Zephyr G3 NGS
  • Freedom EVO NGS
TruSeq Stranded Total RNA
  • Biomek i7
  • epMotion 5075t
  • NGS STAR
  • Sciclone G3 NGSx
Sciclone G3 NGSx iQ
Zephyr G3 NGS
  • Freedom EVO NGS
DreamPrep
TruSight Oncology 500 Biomek i5
  • Biomek i7
  • Biomek NGeniuS
 epMotion 5075t
  • NGS STAR
  • NGS STARlet
  • Fontus NGS
  • Sciclone G3 NGSx
 DreamPrep
TruSight Oncology 500 ctDNA v2 Ìý Ìý
  • STAR MOA
 Ìý Ìý
TruSight Oncology 500 (DNA Only) Biomek i5
Biomek i7
  • Biomek NGeniuS
 Ìý Ìý Ìý Ìý
TruSight Oncology 500 High-Throughput
  • Biomek i7
 Ìý
  • STAR MOA
 Ìý DreamPrep
TruSight RNA Pan-Cancer Panel
  • Biomek i7
 epMotion 5075t Ìý Ìý Ìý
TruSight Tumor 15 Ìý
  • epMotion 5075t
 Ìý Ìý Ìý
TruSight Tumor 170
  • Biomek i5
 Ìý
  • NGS STAR
NGS STARlet		 Ìý Ìý

Starting in January 2022, Hamilton will refer to the NGS STAR configuration for TruSight Oncology library preparations as the SBS STAR (LSA). All existing VRÕæÈ˲ÊƱ Qualified methods are backwards compatible and will continue to run on this system as this change is in name only.

** In order to accommodate for higher dead volume requirements associated with automated platforms and variation in overfill volumes by the original reagent manufacturer, more than one AmpliSeq for VRÕæÈ˲ÊƱ kit may be required when preparing the maximum number of libraries per kit.

Featured automation workflow: whole-genome sequencing

Track samples, manage your runs, and launch into production with preset laboratory information management system (LIMS) protocols. Leverage a compatible robotic liquid handler to prepare libraries for rapid high-throughput sequencing and data analysis.

1
Manage workflow

Clarity LIMS

Laboratory information management system designed to track samples and manage workflows for an optimized and efficient lab. 

2
Prepare libraries

VRÕæÈ˲ÊƱ DNA PCR-Free Prep

High-performing, fast, and integrated PCR-free workflow for sensitive applications such as human whole-genome sequencing or tumor–normal sequencing.

Beckman Coulter or , Hamilton , or another compatible robotic liquid handler.

3
Sequence

NovaSeq X Series

Sequencing systems delivering up to 16 Tb of output, allowing sequencing of 128 human genomes at 30× coverage in a single run.

NovaSeq 6000 System

Scalable throughput and flexibility for virtually any genome, sequencing method, and scale of project.

4
Analyze

VRÕæÈ˲ÊƱ DRAGEN secondary analysis

Accurate, comprehensive, and efficient secondary analysis of next-generation sequencing data.

Emedgene

A comprehensive research software platform to support tertiary analysis clinical research workflows.  

Automation platform info and documentation

Learn more about the automated liquid-handling platforms offered by VRÕæÈ˲ÊƱ partners. View partner documentation to learn how to automate a specific VRÕæÈ˲ÊƱ library prep kit.

Related resources

Adopt high-throughput sequencing in your lab

How to choose a LIMS for NGS

Multiplex sequencing strategies

Bioinformatics pipeline considerations

Automated library prep examples

COVIDSeq workflow automation

COVIDSeq Assay and COVIDSeq Test (RUO) workflows are compatible with a range of automation solutions and configurable for batch sizes from 8–96 samples.

Need more info?

Contact us to learn more about automation partners and VRÕæÈ˲ÊƱ Qualified methods.